Microbial load and microflora of "chat" (Cata edulis Forsk) and effect of "chat" juice on some foodborne pathogens

Abstract

Abstract:

Chewable leaves of "chat" (Cata edulis) were collected from retail shops in Awassa and analyzed for their microbial load and microflora in March - June, 1995. Aerobic mesophilic bacteria, Enterobacteriaceae and bacterial spores had mean counts of >104 cfu/g. Staphylococcus sp., Bacillus sp. and yeasts and molds had mean counts of 103 cfu/g. Between 60% and 80% of the "chat" samples yielded Enterobacteriaceae, Bacillus sp., Micrococcus sp. and Staphylococcus sp. The aerobic mesophilic flora was dominated by Enterobacteriaceae (25%), Bacillus sp. (22%), Micrococcus sp. (18%) and Staphylococcus sp. (13%). About 47% of the Staphylococcus isolates were Staphylococcus aureus. "Chat" juice retarded growth of Salmonella typhimurium, Salmonella enteritidis and Staphylococcus aureus at higher concentrations, but complete inhibition was not attained even at 100% concentration. Bacillus cereus was inhibited at lower concentrations (25%). Inhibition of Listeria monocytogenes was observed only at 100% concentrations. [Ethiop. J. Health Dev. 1997;11(1):83-87]