ANTIFUNGAL METABOLITES FROM SUBMERGED CULTURE OF GANODERMA LUCIDUM (POLYPORE)

Abstract

ABSTRACT:

About 60 different basidiomycete cultures were screened for antimicrobial secondary metabolites. Among basidiomycetes screened for antimicrobial activity, the culture filtrate extract of the polypore, G. lucidum produced the most effective antifungal compounds. Growth in submerged culture of the polypore and isolation methods of the two antifungal antibiotics are described. These compounds were eleased to the culture fluid and the maximum amount of antifungal compounds was obtained after 12 days of submerged growth at 120 revolution per minute (rpm). The culture filtrate were characterized biologically. These metabolites had a wide spectrum of antifungal activity and affected the growth of several saprophytic as well as pathogenic fungi. The minimal inhibitory concentration (MIC) of 201A against Candida albicans and Candida pseudotropicalis was less than 1 mcg/m1 and 1-5 mcg/m1 respectively. Inhibition diameter zone of 36 mm was produced when 10 mcg/disc of 201A was applied on agar medium seeded with Aspergillus flavus .Bacteria were affected only at high concentration. Ethiop.J. Health Dev. 1994;8(1):63-70]